pfge chromosome size standards Search Results


dna marker  (New England Biolabs)
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Dna Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast chromosome pfg marker  (New England Biolabs)
86
New England Biolabs yeast chromosome pfg marker
Yeast Chromosome Pfg Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pfge chromosome size standards  (Bio-Rad)
90
Bio-Rad pfge chromosome size standards
Pfge Chromosome Size Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast chromosome pfg markers  (New England Biolabs)
86
New England Biolabs yeast chromosome pfg markers
Yeast Chromosome Pfg Markers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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saccharomyces cerevisiae chromosome pfge markers  (New England Biolabs)
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New England Biolabs saccharomyces cerevisiae chromosome pfge markers
Saccharomyces Cerevisiae Chromosome Pfge Markers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast chromosome pfge marker  (Millipore)
90
Millipore yeast chromosome pfge marker
Yeast Chromosome Pfge Marker, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chef-dr-iii pulse field gel electrophoresis (pfge) system  (Bio-Rad)
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Bio-Rad chef-dr-iii pulse field gel electrophoresis (pfge) system
Chef Dr Iii Pulse Field Gel Electrophoresis (Pfge) System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast chromosome pfg markers  (Bio-Rad)
90
Bio-Rad yeast chromosome pfg markers
Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the <t>PFG</t> shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei <t>(Hw)</t> <t>chromosomes</t> ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.
Yeast Chromosome Pfg Markers, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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saccharomyces cerevisiae chromosomes  (New England Biolabs)
86
New England Biolabs saccharomyces cerevisiae chromosomes
Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the <t>PFG</t> shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei <t>(Hw)</t> <t>chromosomes</t> ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.
Saccharomyces Cerevisiae Chromosomes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s lambda pfg dna markers  (New England Biolabs)
96
New England Biolabs s lambda pfg dna markers
Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the <t>PFG</t> shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei <t>(Hw)</t> <t>chromosomes</t> ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.
S Lambda Pfg Dna Markers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda dna marker for pfge (lambda ladder)  (Bio-Rad)
90
Bio-Rad lambda dna marker for pfge (lambda ladder)
Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the <t>PFG</t> shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei <t>(Hw)</t> <t>chromosomes</t> ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.
Lambda Dna Marker For Pfge (Lambda Ladder), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the PFG shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei (Hw) chromosomes ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.

Journal: Nucleic Acids Research

Article Title: CRISPR-mediated isolation of specific megabase segments of genomic DNA

doi: 10.1093/nar/gkx749

Figure Lengend Snippet: Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the PFG shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei (Hw) chromosomes ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.

Article Snippet: Size ladders of yeast chromosomes or the Yeast Chromosome PFG Markers (Bio-Rad) are loaded on all megabase resolution gels.

Techniques: In Vitro, CRISPR, Staining, Southern Blot, Labeling